Team II Functional Annotation Group

Team 2: Functional Annotation

Team Members: Danielle Temples, Courtney Astore, Rhiya Sharma, Ujani Hazra, Sooyoun Oh

Introduction

What is Functional Annotation?

The practice of putting biological meaning to coding genes (genes that encode proteins) and their corresponding protein sequences. Such annotations can be derived using homology and ab initio based approaches, which will be further explained in subsequent sections.

Objective: Perform a full functional annotation on the genes and proteins determined by the Gene Prediction group that is relevant to C. jejuni

Homology Approaches

• Determine function via sequence similarity to already functionally annotated sequences
• Limited by what we already know.

Ab Initio Approaches

• Determine function via predictive model without comparing to existing sequences
• Based on laws of nature
• Difficult to verify without experiments

Data Overview

We received 50 fna and 50 faa files from the gene prediction group. The 50 fna files are multifasta files representing each genome. The 50 faa files are multifasta files representing each proteome.